10L/30L/50L Pilot Scale SIP Fermentation System
Product
The 10L / 30L / 50L SIP Pilot Scale Fermentation Systems allows you to run small volume of fermentation experiments with ease. These systems are perfectly suitable for small scaling-up in the academic lab. The easy programming and operation on our Winpact software makes your operation procedure in your peace of mind. This fermenter comes with rolling wheels for mobility. All materials are made from the highest standard to ensure optimization of your fermentation process. A variety of optional devices are also available to maintain your optimum performance.
A. Harvest valve: sterilizable, diaphragm-type tank bottom valve for sanitary post-process harvest
B. Sampling valve: steam-sterilizable valve permits repeatedly sanitary sample collection
B1.Motor - Manual or automatic control of constant agitation speed.
B2.Stainless Steel - Vessel Special designed heating jacket provides better heating efficiency
B3.Peristaltic Pump - Uses four Watson Marlow built-in peristaltic pumps for all your feeding needs
B4.Control Station - Large screen and graphical user interface
Major Science SIP Fermentation Systems
- Wide range of vessel selection, from 10L to 50L working volume
- Colorful interface at 10.4" or above
- Fully automated process with remote monitoring
- 15-step automatic program setting
- Orbital welding ensures minimal residue buildup
- Highest grade construction with 316L stainless steel
- Jacket design provides astounding temperature control
- Exhaust pressure relief valve for maximum safety precaution
- Multiple safety design integration for peace of mind operation
- Remote monitoring & controlling software free from purchase
- Password protection for multiple users with special requirements
- Various optional devices for process optimization and needs
- Ethernet communication with Winpact SCADA software, and IP address
 |
 |
 |
| Four-staged DO cascade | ||
 |
||
| 15-Step programmable PID control | Immediate visualization on operation overview | Easy and intuitive operation for manual and sequence control |
 |
 |
 |
| One-Touch automatic sterilization for vessel and system tubing | Online system calibration with system feedback | System expansion with various optional devices |
* Technical specification subject to change without notice.
* All images are for reference only, actual products might differ from the pictures above.
- * User-friendly, graphical control interface
- * Includes secure user accounts, with different levels of access
- * Modularized and standardized design (Module Skid): ergonomically designed according to height, ease of vessel clean up, and ease of extraction in relation to vessel bottom valve
- * Includes maintenance page with system diagnostics
- * All programmed setting values are automatically stored into the built-in memory; the settings will not be lost in case of power outage/interruption. When power is restored, the interrupted process will automatically resume
| Capacity | 10L | 30L | 50L |
|---|---|---|---|
| Total volume | 15L | 42L | 67L |
| Working volume | 10L | 30L | 50L |
| Vessel and jacket maximum working pressure | 3 bar (43.5 psig) / 4 bar (58 psig) | ||
| Type | Double layered fully enclosed capsule-type tank | ||
| Material | Direct contact to medium - 316L stainless steel; all others - 304 stainless steel | ||
| Surface finish | Interior polish ≦ 25 Ra/in (0.6 μm) Mechanical polishing; Electropolish optional Exterior polish ≦ 32 Ra/in (0.8 μm) Mechanical polishing; Electropolish optional |
||
| Ports | Ports designed according to user requirements | ||
| Piping and valve materials | Parts that directly contact with the product/medium uses 316L stainless steel (≤25 Ra/in) internal polished tubing (BPE standard) :
A. Constructed with 304 stainless steel |
||
| Controller | 10.4” color industrial touch screen | ||
| Setting | * Automated sterilization process * Automated fermentation program |
||
| DO |
|
||
| pH |
|
||
| Pump |
|
||
| Temperature |
|
||
| Agitation |
|
||
| Air supply and exhaust | Gas supply and dehumidifer: uses in-house air compressor or air dehumidifier
|
||
Utility Requirement
| Capacity | 10L | 30L | 50L |
|---|---|---|---|
| Power | Three phase 220V or 380V (note: can be customized to local standard) | ||
| Air | At least 6 bar | ||
| 30 L/min flow rate | 90 L/min flow rate | 150 L/min flow rate | |
| Dehumidified | |||
| Oil-free | |||
| Peripheral factory water supply | Cooling water (tap water, at least 15oC below working temperature, must be soft water) ; Pressure at least 2 bar |
||
| Process water | RO Water | ||
| Plant steam | ≥ 2 bar; dry steam with no entrained condensate | ||
| Process steam | ≥ 2 bar; dry steam with no entrained condensate | ||
| Drain | In situ drain; ≥ 1" | ||
*Customization on the SIP Fermentation system available upon request. Please contact your regional manager for evaluation request.
Optional Accessories Items
Gas Mixing Station
The gas mixing station allows the user to optimize cell growth conditions by independently supplying up to four gasses to the fermentation process. Parameters such as dissolved oxygen and pH can be controlled by adjusting the gas composition supplied to the system. Four manually adjusted flowmeters control the flow rate of each gas, while the 4 solenoid valves automatically open or close in response to the culture conditions. The Gas Mixing Station can be operated in either manual or automatic modes.
Oxygen Mass Flow Controller
Maintain optimal control over culture DO level by installing this optional mass flow controller. The mass flow controller can accurately adjust the flow rate of incoming oxygen and is resistant to fluctuations in gas pressure, ensuring precise control and repeatibility of experimental conditions
- A. Cascade control scheme
- B. Integrated into controller for simple and automated operation
CO2 / O2 Off-Gas Analyzer
The CO2 / O2 off-gas analyzer provides real-time measurement of carbon dioxide and oxygen concentration of the bioreactor exhaust gas. The CO2 concentration is determined using a self-calibrating non-dispersion infrared sensor, while an electrochemical sensor monitors the oxygen concentration. Using this information, the user can continuously monitor metabolism and analyze cell growth parameters.
Cell Density Monitor
Our special implemented online cell density device allows you to obtain direct information about your cell growth rate and cell density; as these values are critical to many bacterial, yeast and animal cell cultures. Online cell density device combines monitoring and probing system, also performs accuracy precisely. Having trouble determining the cell growth rate? Our online cell density is the key to your solution.
ORP Probe
The ORP probe measures the oxidation-reduction potential of the fermentation media, which is a crucial indicator of anaerobic conditions/reactions. This low-maintenance and sterilizable probe is designed to withstand repeated experiments.
Electropolish (EP) of Vessel Tank
Electropolish of the vessel tank is offered as a higher sanitary grade surface finish. EP surface finish is an addition to the standard mechanical polish (MP) which provides a smoother surface area to minimize residual residue.
* Note that this option MUST be requested at your initial inquiry, later-on additions after completion of construction is not possible
Transfer Piping
Transfer piping of your resulting product/medium between vessels is offered for the convenience of operation.
Automated transfer using pressure in addition to directional control with valve regulations offers fast and easy operation.
Turbidity Probe
The turbidity probe measures the turbidity level of the fermentation media, which provides you an indication for the metabolic reaction and in terms allows you to maintain a steady cell level.
Load Cell
The load cell provides weighing capability to your fermentation process. Weighing the substrate feeding can indicate how much substrate was added during the fermentation process, which allows the user to provide more accurate control accordingly to what the process needs.
Operation Platform
An operation platform is offered to provide optimal operation convenience. Stainless steel constructed platform offers elevated height for full observation of the headplate when standing on the platform, the chest-leveled positioning of the vessel headplate prevents falling into the vessel.